Search results for " agglutination"

showing 5 items of 5 documents

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)

2017

Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensors for capturing and identifying bacterial species. In this work, three C-type lectins from the Lepidoptera Spodoptera exigua were produced as recombinant proteins and their bacterial agglutination properties were characterized. The lowest protein concentration producing bacterial agglutination against a panel of different Gram+ an…

0301 basic medicineagglutinationlcsh:BiotechnologyClinical BiochemistryMicrobial Sensitivity TestsSpodopteraSpodopteraC-type lectin; agglutination; CRD; bacterial detection; <i>E. coli</i>ArticleMicrobiologylaw.inventionbacterial detectionLepidoptera genitalia03 medical and health sciences0302 clinical medicinelawC-type lectinlcsh:TP248.13-248.65Agglutination TestsExiguaEscherichia coliAnimalsLectins C-TypeAmino Acid SequenceConserved SequencePhylogenybiologyE. coliCRDGeneral Medicinebiology.organism_classificationImmunity InnateC-type lectinAgglutination (biology)030104 developmental biologyRecombinant DNANoctuidaeInsect ProteinsProtein concentration030215 immunologybiotechnology
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Toxoplasma gondii seroprevalence varies by cat breed.

2017

Toxoplasma gondii is a widespread zoonotic parasite that is relevant for veterinary and public health. The domestic cat, the definitive host species with the largest worldwide population, has become evolutionarily and epidemiologically the most important host of T. gondii. The outcome of T. gondii infection is influenced by congenital and acquired host characteristics. We detected differences in T. gondii seroprevalence by cat breed in our previous studies. The aims of this study were to estimate T. gondii seroprevalence in selected domestic cat breeds, and to evaluate whether being of a certain breed is associated with T. gondii seropositivity, when the age and lifestyle of the cat are tak…

Male0301 basic medicineVeterinary medicineHOSTPhysiologylcsh:MedicinePathogenesis413 Veterinary sciencePathology and Laboratory MedicineBiochemistryDISEASEToxoplasma Gondii0403 veterinary scienceAnimal ProductsSeroepidemiologic StudiesImmune PhysiologyDirect agglutination testMedicine and Health SciencesEthnicitieslcsh:ScienceMammalsProtozoanseducation.field_of_studyImmune System ProteinsMultidisciplinarybiologyFATAL TOXOPLASMOSISAge FactorsAgriculture04 agricultural and veterinary sciencesFINLANDSERIESBreedFELINE INFECTIOUS PERITONITISPREVALENCE3. Good healthVertebratesHost-Pathogen InteractionsFemaleOTOCOLOBUS-MANULToxoplasmaResearch ArticleMeatNorwegian People040301 veterinary sciencesAnimal TypesImmunologyPopulationbiology.animal_breedAntibodies03 medical and health sciencesParasite Groupsparasitic diseasesmedicineAnimalsSeroprevalenceDomestic AnimalseducationNutritionlcsh:ROrganismsOocystsBiology and Life SciencesProteinsToxoplasma gondiiNorwegian Forest catbiology.organism_classificationmedicine.diseaseParasitic ProtozoansFeline infectious peritonitisToxoplasmosisDietLogistic ModelsToxoplasmosis Animal030104 developmental biologyFoodAmniotesPeople and PlacesMultivariate AnalysisCatsPopulation GroupingsParasitologylcsh:QZoologyApicomplexaPLoS ONE
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F-type lectin from serum of the Antarctic teleost fish Trematomus bernacchii (Boulenger, 1902): Purification, structural characterization, and bacter…

2021

Abstract The increasing availability of sequenced genomes has enabled a deeper understanding of the complexity of fish lectin repertoires involved in early development and immune recognition. The teleost fucose-type lectin (FTL) family includes proteins that preferentially bind fucose and display tandemly arrayed carbohydrate-recognition domains (CRDs) or are found in mosaic combinations with other domains. They function as opsonins, promoting phagocytosis and the clearance of microbial pathogens. The Antarctic fish Trematomus bernacchii is a Perciforme living at extremely low temperatures (−1.68 °C) which is considered a model for studying adaptability to the variability of environmental w…

PhysiologyAntarctic fishLectins F-type lectin Antarctic fish Trematomus bernacchii Bacterial agglutinationAntarctic RegionsTrematomus bernacchiiBiochemistryAntarctic fish; Bacterial agglutination; F-type lectin; Lectins; Trematomus bernacchii; Amino Acid Sequence; Animals; Antarctic Regions; Bacteria; Base Sequence; Fucose; Lectins; Perciformes; PhylogenyFucose03 medical and health scienceschemistry.chemical_compound0302 clinical medicineAffinity chromatographyWestern blotLectinsTrematomusmedicineAnimalsAmino Acid SequenceSea bassPerciformeMolecular BiologyOpsoninPhylogeny030304 developmental biologyFucoseAntarctic Region0303 health sciencesbiologyMolecular massmedicine.diagnostic_testBacteriaBase SequenceAnimalLectinBacterial agglutinationbiology.organism_classificationPerciformesBiochemistrychemistrybiology.proteinLectinF-type lectin030215 immunologyComparative biochemistry and physiology. Part B, Biochemistrymolecular biology
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The Role of Lectins in Finfish: A Review

2019

The immune system of vertebrates involves both innate and acquired immune responses. The innate immunity is more generalized with robust response whereas the other has a highly specific response to infectious pathogens. Because of the lack of specialized lymphatic organs, innate immunity is an important mode of defense in fishes. The less specific innate immune system acts mainly through complement pathway which depends on pattern-based recognition of “self” and “non-self” targets by host lectins and associated proteins. This ultimately results in the clearance of target cells. Lectins are glycoproteins which possess at least one carbohydrate recognition domain (CRD) that specifically and r…

chemistry.chemical_classificationInnate immune systemPathogen-associated molecular patternPhagocytosischemical and pharmacologic phenomenabiochemical phenomena metabolism and nutritionManagement Monitoring Policy and LawAquatic ScienceBiologyLectins carbohydrate recognition domain innate immune response bacterial agglutination pathogen-associated molecular patternsComplement systemCell biologyImmune systemLymphatic systemchemistrybacteriaCell adhesionGlycoproteinEcology Evolution Behavior and Systematics
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Primary structure and opsonic activity of an F-lectin from serum of the gilt head bream Sparus aurata (Pisces, Sparidae)

2012

lectin Fucose agglutination fish sequence
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